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THE EFFECTS OF ACRYLAMIDE ON β‐GLUCURONIDASE AND ACID PHOSPHATASE ACTIVITIES IN RAT SCIATIC NERVE ABOVE AND BELOW A LIGATURE
Author(s) -
CAVANAGH J. B.,
NOLAN C. C.
Publication year - 1982
Publication title -
neuropathology and applied neurobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.538
H-Index - 95
eISSN - 1365-2990
pISSN - 0305-1846
DOI - 10.1111/j.1365-2990.1982.tb00314.x
Subject(s) - ligature , acrylamide , sciatic nerve , acid phosphatase , chemistry , alkaline phosphatase , endocrinology , enzyme , medicine , biochemistry , organic chemistry , copolymer , polymer
Cavanagh J.B. & Nolan C.C. 1982 Neuropathology and Applied Neurobiology 8, 465–476 The effects of acrylamide on β‐glucuronidase and acid phosphatase activities in rat sciatic nerve above and below a ligature The enzymes β‐glucuronidase and acid phosphatase have been assayed by histochemical and by fluorimetric methods above and below a tightly tied ligature on the rat sciatic nerve over the subsequent 10 days. These findings have been compared with similarly treated animals also given four daily doses of acrylamide (50 mg/kg). The following have been found: 1 during this time, acrylamide at this dose causes slight increases in β‐glucuronidase in untied sciatic nerves, detectable both histochemically and fluorimetrically; 2 below the ligature both enzyme activities were greatly increased and this was slightly reduced in the acrylamide‐dosed animals; 3 there was a mild rise in β‐glucuronidase activity in the 10 cm above the ligature in undosed animals demonstrable both histochemically and fluorimetrically; 4 that in the acrylamide‐dosed animals there was a marked rise in β‐glucuronidase activity both 10 cm and 20 cm above the ligature which was intensified at 7 days and at 10 days after dosing. This was demonstrated both histochemically and fluorimetrically. These results are discussed in the light of the known sheath cell and axonal responses to acrylamide intoxication.