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EFFECTS OF METHYLMERCURY ON IN VIVO PROTEIN SYNTHESIS IN ISOLATED CEREBRAL AND CEREBELLAR NEURONS
Author(s) -
SYVERSEN T. L. M.
Publication year - 1977
Publication title -
neuropathology and applied neurobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.538
H-Index - 95
eISSN - 1365-2990
pISSN - 0305-1846
DOI - 10.1111/j.1365-2990.1977.tb00586.x
Subject(s) - methylmercury , cerebellum , in vivo , neuron , purkinje cell , chemistry , cell , biology , in vitro , biochemistry , neuroscience , microbiology and biotechnology , genetics , selenium , organic chemistry
Effects of methylmercury on in vivo protein synthesis in isolated cerebral and cerebellar neurons A preparative method is described for the bulk isolation of two neuronal populations from cerebellum, namely the granule cell and Purkinje cell bodies. This isolation method was used for studying in vivo effects of methylmercury on protein synthesis. The experimental rats were given a single i.p. dose of 10 mg/kg methylmercury. Mercury levels are reported in blood, total brain, cerebral neurons and two cerebellar neuron populations. The methylmercury was shown to inhibit incorporation of radioactive leucine into protein. The time course and degree of inhibition were dependent on cell type. The highest inhibition was found in neurons which are morphologically the most mercury sensitive species of the cells tested.