Premium
EXPERIMENTAL STUDIES ON THE POSTNATAL DEVELOPMENT OF THE BRAIN. III. CEREBELLAR DEVELOPMENT FOLLOWING LOCALIZED ADMINISTRATION OF ENU
Author(s) -
DAS G. D.,
PFAFFENROTH M. J.
Publication year - 1977
Publication title -
neuropathology and applied neurobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.538
H-Index - 95
eISSN - 1365-2990
pISSN - 0305-1846
DOI - 10.1111/j.1365-2990.1977.tb00583.x
Subject(s) - cerebellum , granular layer , mitosis , regeneration (biology) , deep cerebellar nuclei , biology , cerebellar cortex , anatomy , microbiology and biotechnology , neuroscience
Experimental studies on the postnatal development of the train. III. Cere‐bellar development following localized administration of ENU Microquantities of N‐ethyl‐N‐nitrosourea (ENU) were injected directly into the cerebellum of 5 and 10 day old rats. It was found that ENU had cytotoxic effects not only on the mitotically active cells of the external granular layer, but also on the non‐mitotic differentiating neurons, including Purkinje cells. Regeneration of the external granular layer, in part due to initial immigration of cells from the normal neighboring folia followed by mitosis, was found to be related to and followed by the recovery of the surviving Purkinje cells. These findings suggested that (a) disorientation and disorganization of the Purkinje cells were due to the treatment and not to the degenerative and regenerative activities of the external granular layer, and that (b) regeneration and increase in the external granular layer resulted from induction by the surviving Purkinje cells. Although microscopically the cerebellum appeared to have regenerated, macroscopically ENU had left lasting effects. The folia, where the carcinogen was injected, showed an absence of secondary folia, and the cerebellum as a whole was small in size.