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THE GROWTH OF TWO SSPE VIRUSES IN ORGANOTYPIC CULTURES OF HAMSTER DORSAL ROOT GANGLIA
Author(s) -
ECOBJOHNSTON MARION
Publication year - 1977
Publication title -
neuropathology and applied neurobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.538
H-Index - 95
eISSN - 1365-2990
pISSN - 0305-1846
DOI - 10.1111/j.1365-2990.1977.tb00574.x
Subject(s) - subacute sclerosing panencephalitis , biology , hamster , measles virus , virus , virology , immunofluorescence , pathology , viral replication , measles , immunology , microbiology and biotechnology , antibody , medicine , vaccination
Two isolates of subacute sclerosing panencephalitis virus (LEC and HallB) were used to infect organotypic cultures of hamster dorsal root ganglia. Virus replication was assessed using indirect immunofluorescence, histological staining, and virus production. Using these criteria, the LEC isolate did not infect the cultures, but the Hallé virus establihed a productive infection. Schwann cells appeared to be the initial site of infection from where virus spread to every cell in the culture by 11 days post‐inoculation (p.i.), except the neurons and their associated satellite cells, both of which contained no antigen and appeared histologically to be the same as controls even at 15 days p.i. The results are discussed with reference to the growth of measles virus in hamster dorsal root ganglia and the usefulness of this system as a model for subacute sclerosing panencephalitis.