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OSMOTIC OPENING OF PERINEURIAL DIFFUSION BARRIER IN PERIPHERAL NERVE
Author(s) -
KRISTENSSON K.,
OLSSON Y.
Publication year - 1976
Publication title -
neuropathology and applied neurobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.538
H-Index - 95
eISSN - 1365-2990
pISSN - 0305-1846
DOI - 10.1111/j.1365-2990.1976.tb00522.x
Subject(s) - horseradish peroxidase , perineurium , tonicity , chemistry , anatomy , in vivo , permeability (electromagnetism) , sciatic nerve , peripheral nervous system , biophysics , pathology , biology , peripheral nerve , central nervous system , medicine , membrane , endocrinology , enzyme , biochemistry , microbiology and biotechnology
The effects of hyperosmolar solutions (sodium chloride and urea) on the perineurial diffusion barrier were tested in experiments using adult mouse sciatic nerves. The solutions were either injected around the nerves in living mice or applied to nerves from killed animals. Thereafter horseradish peroxidase (HRP) was injected around the nerves in order to detect any changes in permeability. HRP was visualized in frozen sections by an enzyme histochemical technique. Hypertonic solutions both in vivo and in vitro caused a pronounced increased permeability of the perineurial diffusion barrier, which persisted for at least one month after injection of 20% NaCl in living mice. In other experiments a hypertonic solution (20% NaCl) was injected one day prior to intradermal inoculation of herpes simplex virus into one hind limb. This treatment dramatically increased the susceptibility of the mice to the infection. This virus has the capacity to spread along nerves to the central nervous system from the site of injection, and is probably taken up at the periphery and then transported centrally inside axons to the cell bodies. The hypothesis is put forward that an important factor for this increased susceptibility is that virions may have an easier access to available surfaces of the nerve fibres due to osmotic opening of the perineurium.