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SUBCUTANEOUS C‐1300 MURINE NEUROBLASTOMA. A LIGHT AND ULTRASTRUCTURAL STUDY
Author(s) -
GRAHAM D. I.,
GONATAS N. K.
Publication year - 1976
Publication title -
neuropathology and applied neurobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.538
H-Index - 95
eISSN - 1365-2990
pISSN - 0305-1846
DOI - 10.1111/j.1365-2990.1976.tb00519.x
Subject(s) - neurofilament , neurite , tissue culture , ultrastructure , cell culture , biology , cell type , neuroblastoma , ribosome , extracellular , budding , golgi apparatus , cell , pathology , chemistry , microbiology and biotechnology , anatomy , in vitro , immunology , medicine , immunohistochemistry , rna , biochemistry , genetics , gene
A light and electron microscopic study has been carried out on the subcutaneous C‐1300 murine neuroblastoma and compared with the morphology of the same tumour grown in both suspension (‘undifferentiated’) and monolayer (‘differentiated’) tissue culture. Two principal cell types, as in tissue culture, were seen in the solid tumour, (a) ‘undifferentiated’ round cells characterized by short microvilli, dispersed ribosomes, scanty neurofilaments and neurotubules and a high mitotic figure rate, and (b) ‘differentiated’ cells characterized by long processes (neurites), aggregation of ribosomes into polysomes, an extensive network of neurofilaments and neurotubules and an occasional dense‐core vesicle. An occasional point of contact between adjacent cells was seen but never synapse formation. These appearances therefore suggest that the subcutaneous tumour cells express some of the differentiated features of the normal cell type from which they are derived. The majority of the cells both in culture and the subcutaneous tumour contained multiple virus‐like A‐type particles. In addition, and possibly of greater significance, was the finding of occasional budding of C‐type particles at the cell surface and the presence of extracellular C‐type particles in the subcutaneous tumour, features which we never saw in the tissue culture material from which the solid neuroblastoma was derived. Possible reasons for this apparent discrepancy are discussed.