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Increased prokineticin 2 expression in gut inflammation: role in visceral pain and intestinal ion transport
Author(s) -
Watson Robert P.,
Lilley Elliot,
Panesar Moh,
Bhalay Gurdip,
Langridge Steven,
Tian ShinShay,
McClenaghan Conor,
Ropenga Anna,
Zeng Fanning,
Nash Mark S.
Publication year - 2012
Publication title -
neurogastroenterology and motility
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.489
H-Index - 105
eISSN - 1365-2982
pISSN - 1350-1925
DOI - 10.1111/j.1365-2982.2011.01804.x
Subject(s) - colitis , inflammation , receptor , enteric nervous system , gastrointestinal tract , inflammatory bowel disease , medicine , irritable bowel syndrome , biology , endocrinology , immunology , pharmacology , disease
Background Prokineticin 2 (PROK2) is an inflammatory cytokine‐like molecule expressed predominantly by macrophages and neutrophils infiltrating sites of tissue damage. Given the established role of prokineticin signaling on gastrointestinal function, we have explored Prok2 gene expression in inflammatory conditions of the gastrointestinal tract and assessed the possible consequences on gut physiology. Methods Prokineticin expression was examined in normal and colitic tissues using qPCR and immunohistochemistry. Functional responses to PROK2 were studied using calcium imaging and a novel antagonist, Compound 3, used to determine the role of PROK2 and prokineticin receptors in inflammatory visceral pain and ion transport. Key Results Prok2 gene expression was up‐regulated in biopsy samples from ulcerative colitis patients, and similar elevations were observed in rodent models of inflammatory colitis. Prokineticin receptor 1 (PKR1) was localized to the enteric neurons and extrinsic sensory neurons, whereas Pkr2 expression was restricted to sensory ganglia. In rats, PROK2‐increased intracellular calcium levels in cultured enteric and dorsal root ganglia neurons, which was blocked by Compound 3. Moreover, PROK2 acting at prokineticin receptors stimulated intrinsic neuronally mediated ion transport in rat ileal mucosa. In vivo , Compound 3 reversed intracolonic mustard oil‐induced referred allodynia and TNBS‐induced visceral hypersensitivity, but not non‐inflammatory, stress‐induced visceral pain. Conclusions & Inferences Elevated Prok2 levels, as a consequence of gastrointestinal tract inflammation, induce visceral pain via prokineticin receptors. This observation, together with the finding that PROK2 can modulate intestinal ion transport, raises the possibility that inhibitors of PROK2 signaling may have clinical utility in gastrointestinal disorders, such as irritable bowel syndrome and inflammatory bowel disease.