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Platelet‐derived growth factor signals play critical roles in differentiation of longitudinal smooth muscle cells in mouse embryonic gut
Author(s) -
Kurahashi M.,
Niwa Y.,
Cheng J.,
Ohsaki Y.,
Fujita A.,
Goto H.,
Fujimoto T.,
Torihashi S.
Publication year - 2008
Publication title -
neurogastroenterology and motility
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.489
H-Index - 105
eISSN - 1365-2982
pISSN - 1350-1925
DOI - 10.1111/j.1365-2982.2007.01055.x
Subject(s) - interstitial cell of cajal , platelet derived growth factor receptor , biology , platelet derived growth factor , microbiology and biotechnology , growth factor , embryonic stem cell , in situ hybridization , cellular differentiation , stromal cell , receptor , endocrinology , immunohistochemistry , immunology , cancer research , gene expression , genetics , gene
In the development of mouse gut, longitudinal smooth muscle cells (LMC) and interstitial cells of Cajal (ICC) originate from common precursor cells expressing c‐Kit. Recently, some gastrointestinal stromal tumours, which develop from smooth muscle layers of the gut and have gain‐of‐function mutations of c‐kit , have been reported to have gain‐of‐function mutations of platelet‐derived growth factor (PDGF) receptor α gene. These data raise the possibility that PDGF signalling might be involved in the development of LMC. Therefore, we examined the expression pattern of the PDGF signal family of embryonic gut by immunohistochemistry and in situ hybridization, and investigated the role of PDGF signals in the development of smooth muscle layers in mouse gut using a new organ culture system. During embryonic development, the circular muscle layer expressed PDGF‐A, enteric neurons expressed PDGF‐B and common precursor cells of LMC and ICC expressed both PDGF receptor α and β. The selective PDGF receptor inhibitor AG1295 suppressed the differentiation of LMC in gut explants. We conclude that PDGF signals play critical roles in the differentiation of LMC in mouse embryonic gut.