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Neurotrophin‐3, but not nerve growth factor, promotes survival of human intestinal mast cells
Author(s) -
Lorentz A.,
Hoppe J.,
Worthmann H.,
Gebhardt T.,
Hesse U.,
Bienenstock J.,
Bischoff S. C.
Publication year - 2007
Publication title -
neurogastroenterology and motility
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.489
H-Index - 105
eISSN - 1365-2982
pISSN - 1350-1925
DOI - 10.1111/j.1365-2982.2007.00899.x
Subject(s) - stem cell factor , nerve growth factor , mast cell , histamine , neurotrophin , interleukin 33 , low affinity nerve growth factor receptor , biology , neurotrophin 3 , tryptase , medicine , apoptosis , endocrinology , growth factor , receptor , microbiology and biotechnology , cytokine , immunology , stem cell , neurotrophic factors , haematopoiesis , interleukin , brain derived neurotrophic factor , biochemistry
Neurotrophins are potent regulators of neuronal cell survival and function. Nerve growth factor (NGF) was shown to reduce apoptosis in cord blood‐derived mast cells. Here, we examined the effect of the neurotrophins NGF and neurotrophin (NT)‐3 on survival and mediator release of human intestinal mast cells. Mast cells isolated from normal intestinal tissue were cultured in the presence of NGF, NT‐3, or stem cell factor (SCF) alone or in the presence of SCF together with each neurotrophin. NGF or NT‐3 alone did not promote mast cell survival. In contrast, mast cell recovery was increased twofold when mast cells were cultured with NT‐3 in addition to SCF for 14 days compared with control. Mast cell recovery was further increased following a combined addition of NT‐3, SCF and IL‐4. NT‐3 mediated mast cell growth was dependent on the primary receptor for NT‐3 TrkC. NGF in combination with SCF or with SCF and IL‐4 showed no effect on mast cell survival. Histamine release and histamine content per mast cell remained unchanged, whereas leukotriene C4 release decreased if mast cells were cultured with NGF or NT‐3 in addition to SCF. In summary, NT‐3 affects mature human mast cells by promoting mast cell survival, whereas NGF does not.