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Propulsive Activity of the Isolated Choledochoduodenal Junction of the Guinea Pig
Author(s) -
Vogalis F.,
Bywater R. A. R.,
Taylor G. S.
Publication year - 1989
Publication title -
neurogastroenterology and motility
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.489
H-Index - 105
eISSN - 1365-2982
pISSN - 1350-1925
DOI - 10.1111/j.1365-2982.1989.tb00149.x
Subject(s) - guinea pig , atropine , tetrodotoxin , perfusion , cholecystokinin , medicine , endocrinology , chemistry , bolus (digestion) , anatomy , biology , receptor
The isolated choledochoduodenal junction of the guinea pig exhibited unidirectional propulsive activity when perfused with prewarmed Krebs' solution through the upper common bile duct. The mean perfusion pressure that elicited propulsive contractions was 6.1 ± 0.3 cm water ( n = 20). The mean frequency of propulsive contractions was 19.1 ± 1.2 per minute ( n = 20), and the mean volume of each expelled bolus of perfusate was 17.5 ± 1.6 μl ( n = 16) when superfused with normal Krebs' solution. The propulsive activity was abolished by atropine (1.4 μM) and by tetrodotoxin (3.1 μM). The contractions became nonpropulsive when d‐tubocurarine (140 μM) was added to the perfusate. Cholecystokinin octapeptide (10 nM) significantly decreased the propulsive contractile frequency from 19.1 to 14.3 per minute ( p < 0.001) and increased the mean ejected bolus volume from 17.5 to 25.1 μl ( p < 0.05). These actions of cliolecystokinin octapeptide were markedly depressed by atropine but less so by d‐tubocurarine. The results suggest that the guinea‐pig choledochoduodenal junction acts like a propulsive motor unit under the control of intrinsic motor nerves whose activities may be modulated by hormones such as cholecystokinin octapeptide.