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Reprogramming of carbon metabolism by the transcriptional activators AcuK and AcuM in Aspergillus nidulans
Author(s) -
Suzuki Yumi,
Murray Sandra L.,
Wong Koon Ho,
Davis Meryl A.,
Hynes Michael J.
Publication year - 2012
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.2012.08067.x
Subject(s) - biology , aspergillus nidulans , glyoxylate cycle , isocitrate lyase , biochemistry , gene , phosphoenolpyruvate carboxykinase , promoter , gluconeogenesis , gene expression , malate synthase , transcription factor , transcription (linguistics) , malate dehydrogenase , microbiology and biotechnology , metabolism , enzyme , linguistics , philosophy , mutant
Summary The ability of fungi to use carbon sources metabolized via the TCA cycle requires gluconeogenesis. In Aspergillus nidulans the AcuK and AcuM transcription factors regulate the expression of the gluconeogenic genes acuF , encoding phosphoenolpyruvate carboxykinase, and acuG , encoding fructose‐1,6‐bisphosphatase. Expressed proteins containing the AcuK/AcuM N‐terminal DNA‐binding domains bind together in vitro to motifs containing repeats of CGG separated by seven bases (CCGN7CCG) and the functionality of these sequences was verified in vivo by acuF–lacZ reporter studies. Chromatin immunoprecipitation analysis showed inter‐dependent DNA binding of the proteins to the promoters of gluconeogenic genes in vivo independent of the carbon source. Deletion of the mdhC gene encoding a cytoplasmic/peroxisomal malate dehydrogenase showed that this activity is not essential for gluconeogenesis and indicated that induction of AcuK/AcuM regulated genes might result from malate accumulation. Deletion of the gene for the alternative oxidase did not affect growth on gluconeogenic carbon sources; however, expression was absolutely dependent on AcuK and AcuM. Orthologues of AcuK and AcuM, are present in a wide range of fungal taxa and the CCGN7CCG motif is present in the 5′ of many genes involved in gluconeogenesis indicating a fundamental role for these transcription factors in reprogramming fungal carbon metabolism.

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