Termination troubles in Escherichia coli K12

Summary In this issue of Molecular Microbiology , Schaub and Hayes report that, compared with other enterobacteria, Escherichia coli K12 carries two mutations – one in the prfB gene encoding the release factor RF2, and the other in the rpsG gene encoding r‐protein S7 – that together concur in compromising translation termination at the essential rpsG gene. As a consequence, the growth of E. coli K12 is very sensitive to a further mutation ( rluD ‐ ) that depresses RF2 activity, whereas the growth of its close relative, E. coli B, is not. We tentatively discuss how the K12‐specific mutations in RF2 and S7 might have occurred and why inefficient translation termination at rpsG inhibits growth. The work of Schaub and Hayes illustrates the fact that, due probably to its long history in the laboratory, E. coli K12 has accumulated mutations that sometimes limit its value as a model for studying basic steps in prokaryotic gene expression.