Premium
Hyperosmosis enhances radiation and hydroxyurea resistance of Schizosaccharomyces pombe checkpoint mutants through the spindle checkpoint and delayed cytokinesis
Author(s) -
Alao John P.,
Huis in 't Veld Pim J.,
Buhse Frederike,
Sunnerhagen Per
Publication year - 2010
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.2010.07193.x
Subject(s) - g2 m dna damage checkpoint , schizosaccharomyces pombe , biology , cytokinesis , cell cycle checkpoint , microbiology and biotechnology , mad2 , chek1 , dna damage , schizosaccharomyces , wee1 , spindle checkpoint , mitosis , cell cycle , dna repair , cdc25 , mutant , cyclin dependent kinase 1 , genetics , cell division , spindle apparatus , dna , cell , gene
Summary The DNA damage and stress response pathways interact to regulate cellular responses to genotoxins and environmental stresses. How these pathways interact in Schizosaccharomyces pombe is not well understood. We demonstrate that osmotic stress suppresses the DNA damage sensitivity of checkpoint mutants, and that this occurs through three distinct cell cycle delays. A delay in G2/M is dependent on Srk1. Progression through mitosis is halted by the Mad2‐dependent spindle checkpoint. Finally, cytokinesis is impaired by modulating Cdc25 expression. These three delays, imposed by osmotic stress, together compensate for the loss of checkpoint signalling.