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A Candida albicans ‐specific region of the α‐pheromone receptor plays a selective role in the white cell pheromone response
Author(s) -
Yi Song,
Sahni Nidhi,
Pujol Claude,
Daniels Karla J.,
Srikantha Thyagarajan,
Ma Ning,
Soll David R.
Publication year - 2009
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.2008.06575.x
Subject(s) - biology , pheromone , mating type , candida albicans , signal transduction , microbiology and biotechnology , receptor , secretion , phenotype , locus (genetics) , intracellular , genetics , biochemistry , gene
Summary Candida albicans strains homozygous at the mating type locus can switch from white to opaque, and must do so to mate. Opaque cells then secrete mating pheromones that stimulate opaque cells of opposite mating type to undergo mating. These same pheromones stimulate mating‐incompetent white cells to become cohesive and adhesive, and enhance white cell biofilm development, a pathogenic trait. Stimulation is mediated through the same receptor, G protein complex and mitogen‐activated protein kinase pathway. Here we present evidence that a C. albicans ‐specific 55‐amino‐acid region of the first intracellular loop, IC1, of the α‐pheromone receptor Ste2p, is required for the α‐pheromone response of white cells, but not that of opaque cells. This represents a unique regulatory configuration in which activation of a common pathway by the same ligand, the same receptor and the same signal transduction pathway is dependent on a unique region of an intracellular loop of the common receptor in one of the two responding phenotypes.