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Sinorhizobium meliloti , a bacterium lacking the autoinducer‐2 (AI‐2) synthase, responds to AI‐2 supplied by other bacteria
Author(s) -
Pereira Catarina S.,
McAuley J. Randall,
Taga Michiko E.,
Xavier Karina B.,
Miller Stephen T.
Publication year - 2008
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.2008.06477.x
Subject(s) - sinorhizobium meliloti , biology , operon , autoinducer , virulence , bacteria , mutant , erwinia , gene , microbiology and biotechnology , quorum sensing , biochemistry , genetics
Summary Many bacterial species respond to the quorum‐sensing signal autoinducer‐2 (AI‐2) by regulating different niche‐specific genes. Here, we show that Sinorhizobium meliloti , a plant symbiont lacking the gene for the AI‐2 synthase, while not capable of producing AI‐2 can nonetheless respond to AI‐2 produced by other species. We demonstrate that S. meliloti has a periplasmic binding protein that binds AI‐2. The crystal structure of this protein (here named SmlsrB) with its ligand reveals that it binds (2 R ,4 S )‐2‐methyl‐2,3,3,4‐tetrahydroxytetrahydrofuran ( R ‐THMF), the identical AI‐2 isomer recognized by LsrB of Salmonella typhimurium. The gene encoding SmlsrB is in an operon with orthologues of the lsr genes required for AI‐2 internalization in enteric bacteria. Accordingly, S. meliloti internalizes exogenous AI‐2, and mutants in this operon are defective in AI‐2 internalization. S. meliloti does not gain a metabolic benefit from internalizing AI‐2, suggesting that AI‐2 functions as a signal in S. meliloti . Furthermore, S. meliloti can completely eliminate the AI‐2 secreted by Erwinia carotovora , a plant pathogen shown to use AI‐2 to regulate virulence. Our findings suggest that S. meliloti is capable of ‘eavesdropping’ on the AI‐2 signalling of other species and interfering with AI‐2‐regulated behaviours such as virulence.