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Complex extracellular interactions of proteases and a protease inhibitor influence multicellular development of Streptomyces coelicolor
Author(s) -
Kim Dae Wi,
Hesketh Andy,
Kim Eun Sook,
Song Ju Yeon,
Lee Dae Hoon,
Kim In Seop,
Chater Keith F.,
Lee Kye Joon
Publication year - 2008
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.2008.06471.x
Subject(s) - proteases , biology , extracellular , protease , streptomyces coelicolor , protease inhibitor (pharmacology) , microbiology and biotechnology , biochemistry , streptomyces , gene , genetics , enzyme , virus , mutant , antiretroviral therapy , viral load , bacteria
Summary Streptomyces coelicolor produces an extracellular protease inhibitor protein, STI ( Streptomyces trypsin inhibitor). We show that post‐growth elimination of STI is needed for colonies to develop aerial mycelium efficiently. Inactivation of STI, and thus the normal progression of colony development, at least partly involves an extracellular protease specified by gene SCO5913. Two‐hybrid analysis identified two possible targets of STI inhibition (the products of SCO1355 and SCO5447), both extracellular proteases containing a domain homologous with the P‐domain of eukaryotic convertases, proteases that mediate the processing of many precursors with important cellular or developmental roles. At least the SCO1355 protease is needed for the normal progression of development. Two components of the proposed cascade are dependent on the tRNA for the rare UUA (leucine) codon, which is specified by the developmental gene bldA. A model is presented that links intracellular regulatory events with an extracellular protease cascade to facilitate normal development.