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The Spx paralogue MgsR (YqgZ) controls a subregulon within the general stress response of Bacillus subtilis
Author(s) -
Reder Alexander,
Höper Dirk,
Weinberg Christin,
Gerth Ulf,
Fraunholz Martin,
Hecker Michael
Publication year - 2008
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.2008.06332.x
Subject(s) - regulon , sigma factor , bacillus subtilis , biology , transcriptome , mutant , gene , genetics , regulator , phenotype , gene expression , microbiology and biotechnology , promoter , bacteria
Summary The alternative sigma factor σ B of Bacillus subtilis is responsible for the induction of the large general stress regulon comprising approximately 150–200 genes. YqgZ, a member of the σ B regulon, resembles the global regulator Spx of the diamide stress regulon in B. subtilis . In this work we conducted a comprehensive transcriptome and proteome analysis of the B. subtilis wild‐type 168 and its isogenic Δ sigB and Δ yqgZ mutants following exposure to 4% (v/v) ethanol stress, which led to the characterization of a ‘subregulon’ within the general stress response that is regulated by YqgZ. Activation and induction of σ B are necessary but not sufficient for a full expression of all general stress genes. Expression of 53 genes was found to be positively regulated and the expression of 18 genes was negatively affected by YqgZ. The identification of the negatively regulated group represents a so far uncharacterized regulatory phenomenon observed in the Δ sigB mutant background that can now be attributed to the function of YqgZ. Due to the strict σ B ‐dependent expression of YqgZ it was renamed to MgsR ( m odulator of the g eneral s tress r esponse).