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Mutation in the rel gene of Sorangium cellulosum affects morphological and physiological differentiation
Author(s) -
Knauber Tina,
Doss Sabrina D.,
Gerth Klaus,
Perlova Olena,
Müller Rolf,
TreunerLange Anke
Publication year - 2008
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.2008.06285.x
Subject(s) - biology , mutant , operon , wild type , multicellular organism , gene , norvaline , mutation , genetics , microbiology and biotechnology , amino acid , valine
Summary Interruption of the (p)ppGpp synthetase gene ( rel ) of Sorangium cellulosum So ce56 resulted in loss of ppGpp accumulation after norvaline treatment during exponential growth phase. The rel mutant failed to produce wild‐type levels of the polyketides chivosazol and etnangien in production media. In wild‐type cells expression of the chivosazol biosynthetic operon can be significantly increased by norvaline or α‐methylglucoside. This induction does not occur in the rel mutant. The rel mutant also lost the capability to form multicellular fruiting bodies under nutrient starvation.