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The identity of the transcription +1 position is crucial for changes in gene expression in response to amino acid starvation in Bacillus subtilis
Author(s) -
Krásný Libor,
Tišerová Hana,
Jonák Jiří,
Rejman Dominik,
Šanderová Hana
Publication year - 2008
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.2008.06256.x
Subject(s) - promoter , bacillus subtilis , biology , stringent response , transcription (linguistics) , gtp' , gene , sigma factor , transcription factor , genetics , gene expression , microbiology and biotechnology , biochemistry , escherichia coli , bacteria , enzyme , philosophy , linguistics
Summary We identify here a pattern in the transcription start sites (+1A or +1G) of σ A ‐dependent promoters of genes that are up‐/downregulated in response to amino acid starvation (stringent response) in Bacillus subtilis . Upregulated promoters initiate mostly with ATP and downregulated promoters with GTP. These promoters appear to be sensitive to changes in initiating nucleoside triphosphate concentrations. During the stringent response in B. subtilis , when ATP and GTP levels change reciprocally, the identity of the +1 position (A or G) of these promoters is a factor important in their regulation. Mutations that change the identity of position +1 (A for G and vice versa) change the response of the promoter to amino acid starvation.