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Spatial dissection of the cis ‐ and trans ‐Golgi compartments in the malaria parasite Plasmodium falciparum
Author(s) -
Struck Nicole S.,
Herrmann Susann,
SchmuckBarkmann Iris,
De Souza Dias Suzana,
Haase Silvia,
Cabrera Ana L.,
Treeck Moritz,
Bruns Caroline,
Langer Christine,
Cowman Alan F.,
Marti Matthias,
Spielmann Tobias,
Gilberger Tim W.
Publication year - 2008
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.2008.06125.x
Subject(s) - golgi apparatus , biology , endoplasmic reticulum , plasmodium falciparum , secretory pathway , microbiology and biotechnology , organelle , fusion protein , genetics , gene , malaria , immunology , recombinant dna
Summary The Golgi apparatus forms the heart of the secretory pathway in eukaryotic cells where proteins are modified, processed and sorted. The transport of proteins from the endoplasmic reticulum (ER) to the cis‐ side of the Golgi complex takes place at specialized ER sub‐domains known as transitional ER (tER). We used the Plasmodium falciparum orthologue of Sec13p to analyse tER organization. We show that the distribution of Pf Sec13p is restricted to defined areas of the ER membrane. These foci are juxtaposed to the Golgi apparatus and might represent tER sites. To further analyse cis ‐ to trans ‐Golgi architecture, we generated a double transfectant parasite line that expresses the Golgi marker Golgi reassembly stacking protein (GRASP) as a green fluorescent protein fusion and the trans‐ Golgi marker Rab6 as a DsRed fusion protein. Our data demonstrate that Golgi multiplication is closely linked to tER multiplication, and that parasite maturation is accompanied by the spatial separation of the cis‐ and trans‐ face of this organelle.