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Chromosome segregation control by Escherichia coli ObgE GTPase
Author(s) -
Foti James J.,
Persky Nicole S.,
Ferullo Daniel J.,
Lovett Susan T.
Publication year - 2007
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.2007.05811.x
Subject(s) - biology , seqa protein domain , chromosome segregation , chromosome , escherichia coli , microbiology and biotechnology , genetics , dna replication , small gtpase , dna , origin of replication , gene , signal transduction
Summary Escherichia coli cells depleted of the conserved GTPase, ObgE, show early chromosome‐partitioning defects and accumulate replicated chromosomes in which the terminus regions are colocalized. Cells lacking ObgE continue to initiate replication, with a normal ratio of the origin to terminus. Localization of the SeqA DNA binding protein, normally seen as punctate foci, however, was disturbed. Depletion of ObgE also results in cell filamentation, with polyploid DNA content. Depletion of ObgE did not cause lethality, and cells recovered fully after expression of ObgE was restored. We propose a model in which ObgE is required to license chromosome segregation and subsequent cell cycle events.