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Thiol‐disulphide oxidoreductase modules in the low‐GC Gram‐positive bacteria
Author(s) -
Kouwen Thijs R. H. M.,
Van Der Goot Annemieke,
Dorenbos Ronald,
Winter Theresa,
Antelmann Haike,
Plaisier MarieClaire,
Quax Wim J.,
Van Dijl January Maarten,
Dubois JeanYves F.
Publication year - 2007
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.2007.05707.x
Subject(s) - biology , oxidoreductase , gram , bacteria , thiol , microbiology and biotechnology , biochemistry , genetics , enzyme
Summary Disulphide bond formation catalysed by thiol‐disulphide oxidoreductases (TDORs) is a universally conserved mechanism for stabilizing extracytoplasmic proteins. In Escherichia coli , disulphide bond formation requires a concerted action of distinct TDORs in thiol oxidation and subsequent quinone reduction. TDOR function in other bacteria has remained largely unexplored. Here we focus on TDORs of low‐GC Gram‐positive bacteria, in particular DsbA of Staphylococcus aureus and BdbA‐D of Bacillus subtilis . Phylogenetic analyses reveal that the homologues DsbA and BdbD cluster in distinct groups typical for Staphylococcus and Bacillus species respectively. To compare the function of these TDORs, DsbA was produced in various bdb mutants of B. subtilis . Next, we assessed the ability of DsbA to sustain different TDOR‐dependent processes, including heterologous secretion of E. coli PhoA, competence development and bacteriocin (sublancin 168) production. The results show that DsbA can function in all three processes. While BdbD needs a quinone oxidoreductase for activity, DsbA activity appears to depend on redox‐active medium components. Unexpectedly, both quinone oxidoreductases of B. subtilis are sufficient to sustain production of sublancin. Moreover, DsbA can functionally replace these quinone oxidoreductases in sublancin production. Taken together, our unprecedented findings imply that TDOR systems of low‐GC Gram‐positive bacteria have a modular composition.