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Protection of antiterminator RNA by the transcript elongation complex
Author(s) -
Sloan Sieghild,
Rutkai Edit,
King Rodney A.,
Velikodvorskaya Tatyana,
Weisberg Robert A.
Publication year - 2007
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.2006.05579.x
Subject(s) - antitermination , biology , terminator (solar) , rna , transcription (linguistics) , rnase p , termination factor , rna silencing , rna polymerase , nuclease protection assay , rna editing , microbiology and biotechnology , small nuclear rna , non coding rna , genetics , gene , rna interference , ionosphere , linguistics , philosophy , physics , astronomy
Summary Nascent transcripts encoded by the putL and putR sites of phage HK022 bind the transcript elongation complex and suppress termination at downstream transcription terminators. We report here that the chemical stability of putL RNA is considerably greater than that of the typical Escherichia coli message because the elongation complex protects this RNA from degradation. When binding to the elongation complex was prevented by mutation of either putL or RNA polymerase, RNA stability decreased more than 50‐fold. The functional modification conferred by putL RNA on the elongation complex is also long‐lived: the efficiency of terminator suppression remained high for at least 10 kb from the putL site. We find that RNase III rapidly and efficiently cleaved the transcript just downstream of the putL sequences, but such cleavage changed neither the stability of putL RNA nor the efficiency of antitermination. These results argue that the continuity of the RNA that connects put sequences to the growing point is not required for persistence of the antiterminating modification in vivo .