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H4 acetylation does not replace H3 acetylation in chromatin remodelling and transcription activation of Adr1‐dependent genes
Author(s) -
Agricola Eleonora,
Verdone Loredana,
Di Mauro Ernesto,
Caserta Micaela
Publication year - 2006
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.2006.05451.x
Subject(s) - acetylation , biology , histone h4 , nucleosome , histone , chromatin remodeling , chromatin , histone code , sap30 , histone h3 , microbiology and biotechnology , transcription (linguistics) , biochemistry , gene , linguistics , philosophy
Summary Histone acetylation regulates gene expression. Whether this is caused by a general increase in nucleosome fluidity due to charge neutralization or by a more specific code is still matter of debate. By using a set of glucose‐repressed Adr1‐dependent genes of Saccharomyces cerevisiae , whose transcription was previously shown to require both Gcn5 and Esa1, we asked how changes of histone acetylation patterns at the promoter nucleosomes regulate chromatin remodelling and activation. When the signal of glucose reduction reaches the cells, H4 acetylation is kept constant while an increase of H3 acetylation occurs, in an Adr1‐ and Gcn5‐dependent manner. In cells lacking Gcn5 activity, the H3 acetylation increase does not occur and an unexpected increase of histone H4 acetylation is observed. Nevertheless, chromatin remodelling and transcription activation are impaired, suggesting that acetylation of H3 and H4 histones plays different roles.