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A STE12 homologue of the homothallic ascomycete Sordaria macrospora interacts with the MADS box protein MCM1 and is required for ascosporogenesis
Author(s) -
Nolting Nicole,
Pöggeler Stefanie
Publication year - 2006
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.2006.05415.x
Subject(s) - biology , mads box , genetics , fungal protein , gene , heterothallic , transcription factor , neurospora crassa , mating type , saccharomyces cerevisiae , mutant , arabidopsis
Summary The MADS box protein MCM1 controls diverse developmental processes and is essential for fruiting body formation in the homothallic ascomycete Sordaria macrospora . MADS box proteins derive their regulatory specificity from a wide range of different protein interactions. We have recently shown that the S. macrospora MCM1 is able to interact with the alpha‐domain mating‐type protein SMTA‐1. To further evaluate the functional roles of MCM1, we used the yeast two‐hybrid approach to identify MCM1‐interacting proteins. From this screen, we isolated a protein with a putative N‐terminal homeodomain and C‐terminal C 2 /H 2 ‐Zn 2+ finger domains. The protein is a member of the highly conserved fungal STE12 transcription factor family of proteins and was therefore termed STE12. Furthermore, we demonstrate by means of two‐hybrid and far western analysis that in addition to MCM1, the S. macrospora STE12 protein is able to interact with the mating‐type protein SMTA‐1. Unlike the situation in the closely related heterothallic ascomycete Neurospora crassa , deletion (Δ) of the ste12 gene in S. macrospora neither affects vegetative growth nor fruiting body formation. However, ascus and ascospore development are highly impaired by the Δ ste12 mutation. Our data provide another example of the functional divergence within the fungal STE12 transcription factor family.