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The inflammation‐associated Salmonella SopA is a HECT‐like E3 ubiquitin ligase
Author(s) -
Zhang Ying,
Higashide Wendy M.,
McCormick Beth A.,
Chen Jue,
Zhou Daoguo
Publication year - 2006
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.2006.05407.x
Subject(s) - effector , biology , salmonella , ubiquitin ligase , inflammation , mutant , microbiology and biotechnology , ubiquitin , host (biology) , bacteria , immunology , genetics , gene
Summary Salmonella translocate a group of type III effectors into the host cells to induce entry, promote survival and cause intestinal inflammation. Although the biochemical and cellular mechanisms of how bacterial effectors function inside host cells remain largely unknown, studies have indicated that a likely strategy is to exploit host cellular pathways through functional mimicry. We report here that SopA, a Salmonella type III effector, mimics the mammalian HECT E3 ubiquitin ligase. SopA preferentially uses the host UbcH5a, UbcH5c and UbcH7 as E2s, which are involved in inflammation. Both the wild‐type SopA and the mutant SopA C753S were expressed and translocated at similar levels during the infection of HeLa cells. A Salmonella strain expressing a catalytically incompetent SopA C753S mutant had reduced Salmonella ‐induced polymorphonuclear leukocytes transepithelial migration. We speculate that SopA ubiquitinate bacterial/host proteins involved in Salmonella ‐induced intestinal inflammation.