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Differential modulation of E. coli mRNA abundance by inhibitory proteins that alter the composition of the degradosome
Author(s) -
Gao Junjun,
Lee Kangseok,
Zhao Meng,
Qiu Ji,
Zhan Xiaoming,
Saxena Ankur,
Moore Christopher J.,
Cohen Stanley N.,
Georgiou George
Publication year - 2006
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.2006.05246.x
Subject(s) - biology , degradosome , abundance (ecology) , composition (language) , modulation (music) , messenger rna , inhibitory postsynaptic potential , microbiology and biotechnology , biochemistry , ecology , gene , physics , neuroscience , rna editing , linguistics , philosophy , acoustics
Summary In Escherichia coli the initial step in the processing or decay of many messenger and structural RNAs is mediated by the endonuclease RNase E, which forms the core of a large RNA‐catalysis machine termed the degradosome. Previous experiments have identified a protein that globally modulates RNA abundance by binding to RNase E and regulating its endonucleolytic activity. Here we report the discovery of RraB, which interacts with a different site on RNase E and interferes with cleavage of a different set of transcripts. We show that expression of RraA or RraB in vivo is accompanied by dramatic, distinct, and inhibitor‐specific changes in degradosome composition – and that these are in turn associated with alterations in RNA decay and global transcript abundance profiles that are dissimilar to the profile observed during simple RNase E deficiency. Our results reveal the existence of endonuclease binding proteins that modulate the remodelling of degradosome composition in bacteria and argue that such degradosome remodelling is a mechanism for the differential regulation of RNA cleavages in E. coli .