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The Bacillus subtilis spore coat protein interaction network
Author(s) -
Kim Hosan,
Hahn Marlene,
Grabowski Paul,
McPherson Derrell C.,
Otte Michele M.,
Wang Rong,
Ferguson Caitlin C.,
Eichenberger Patrick,
Driks Adam
Publication year - 2006
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.2005.04968.x
Subject(s) - bacillus subtilis , coat , biology , spore , computational biology , protein–protein interaction , interaction network , microbiology and biotechnology , bacteria , biochemistry , genetics , gene , ecology
Summary Bacterial spores are surrounded by a morphologically complex, mechanically flexible protein coat, which protects the spore from toxic molecules. The interactions among the over 50 proteins that make up the coat remain poorly understood. We have used cell biological and protein biochemical approaches to identify novel coat proteins in Bacillus subtilis and describe the network of their interactions, in order to understand coat assembly and the molecular basis of its protective functions and mechanical properties. Our analysis characterizes the interactions between 32 coat proteins. This detailed view reveals a complex interaction network. A key feature of the network is the importance of a small subset of proteins that direct the assembly of most of the coat. From an analysis of the network topology, we propose a model in which low‐affinity interactions are abundant in the coat and account, to a significant degree, for the coat's mechanical properties as well as structural variation between spores.