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Porphyromonas gingivalis ‐induced platelet aggregation in plasma depends on Hgp44 adhesin but not Rgp proteinase
Author(s) -
Naito Mariko,
Sakai Eiko,
Shi Yixin,
Ideguchi Hiroshi,
Shoji Mikio,
Ohara Naoya,
Yamamoto Kenji,
Nakayama Koji
Publication year - 2006
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.2005.04942.x
Subject(s) - porphyromonas gingivalis , bacterial adhesin , platelet , platelet rich plasma , microbiology and biotechnology , platelet activation , periodontitis , biology , receptor , chemistry , immunology , biochemistry , gene , medicine , escherichia coli
Summary Evidence from recent epidemiological studies suggests a link between periodontal infections and increased risk of atherosclerosis and related cardiovascular and cerebrovascular events in human subjects. One of the major pathogens of periodontitis, Porphyromonas gingivalis, has the ability to aggregate human platelets in platelet‐rich plasma (PRP). Mechanism of P. gingivalis ‐induced platelet aggregation in PRP was investigated. Proteinase inhibitors toward Arg‐gingipain (Rgp) and Lys‐gingipain (Kgp) did not suppress P. gingivalis ‐induced platelet aggregation in PRP, whereas the Rgp inhibitor markedly inhibited P. gingivalis ‐induced platelet aggregation using washed platelets. Mutant analysis revealed that P. gingivalis ‐induced platelet aggregation in PRP depended on Rgp‐, Kgp‐ and haemagglutinin A (HagA)‐encoding genes that intragenically coded for adhesins such as Hgp44. Hgp44 adhesin on the bacterial cell surface, which was processed by Rgp and Kgp proteinases, was essential for P. gingivalis ‐induced platelet aggregation in PRP. P. gingivalis cell‐reactive IgG in plasma, and FcγRIIa receptor and to a lesser extent GPIbα receptor on platelets were found to be a prerequisite for P. gingivalis ‐induced platelet aggregation in PRP. These results reveal a novel mechanism of platelet aggregation by P. gingivalis .