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A deficiency in S‐adenosylmethionine synthetase interrupts assembly of the septal ring in Escherichia coli K‐12
Author(s) -
Wang Shan,
Arends S. J. Ryan,
Weiss David S.,
Newman Elaine B.
Publication year - 2005
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.2005.04864.x
Subject(s) - ftsz , biology , mutant , escherichia coli , ring (chemistry) , microbiology and biotechnology , biochemistry , gene , chemistry , organic chemistry
Summary A mutant in which S‐adenosylmethionine synthetase is underexpressed makes filaments with no visible septa. Examination with GFP fusions to various septal proteins shows that FtsZ, ZipA and FtsA localize to the septal ring, but FtsQ, FtsW, FtsI or FtsN do not. The requirement for S‐adenosylmethionine suggests that some methylation reaction is required before a complete septal ring can be assembled.