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PKA and Sch9 control a molecular switch important for the proper adaptation to nutrient availability
Author(s) -
Roosen Johnny,
Engelen Kristof,
Marchal Kathleen,
Mathys Janick,
Griffioen Gerard,
Cameroni Elisabetta,
Thevelein Johan M.,
De Virgilio Claudio,
De Moor Bart,
Winderickx Joris
Publication year - 2005
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.2004.04429.x
Subject(s) - saccharomyces cerevisiae , biology , effector , yeast , kinase , gene , nutrient sensing , microbiology and biotechnology , phenotype , protein kinase a , gene expression , regulation of gene expression , signalling , signal transduction , genetics , biochemistry
Summary In the yeast Saccharomyces cerevisiae , PKA and Sch9 exert similar physiological roles in response to nutrient availability. However, their functional redundancy complicates to distinguish properly the target genes for both kinases. In this article, we analysed different phenotypic read‐outs. The data unequivocally showed that both kinases act through separate signalling cascades. In addition, genome‐wide expression analysis under conditions and with strains in which either PKA and/or Sch9 signalling was specifically affected, demonstrated that both kinases synergistically or oppositely regulate given gene targets. Unlike PKA, which negatively regulates stress‐responsive element (STRE)‐ and post‐diauxic shift (PDS)‐driven gene expression, Sch9 appears to exert additional positive control on the Rim15‐effector Gis1 to regulate PDS‐driven gene expression. The data presented are consistent with a cyclic AMP (cAMP)‐gating phenomenon recognized in higher eukaryotes consisting of a main gatekeeper, the protein kinase PKA, switching on or off the activities and signals transmitted through primary pathways such as, in case of yeast, the Sch9‐controlled signalling route. This mechanism allows fine‐tuning various nutritional responses in yeast cells, allowing them to adapt metabolism and growth appropriately.