Premium
A small, cysteine‐rich protein secreted by Fusarium oxysporum during colonization of xylem vessels is required for I‐3 ‐mediated resistance in tomato
Author(s) -
Rep Martijn,
Van Der Does H. Charlotte,
Meijer Michiel,
Van Wijk Ringo,
Houterman Petra M.,
Dekker Henk L.,
De Koster Chris G.,
Cornelissen Ben J. C.
Publication year - 2004
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.2004.04177.x
Subject(s) - xylem , biology , fusarium oxysporum , gene , mutant , microbiology and biotechnology , cysteine , virulence , fusarium wilt , genetics , botany , biochemistry , enzyme
Summary A 12 kDa cysteine‐rich protein is secreted by Fusarium oxysporum f. sp. lycopersici during colonization of tomato xylem vessels. Peptide sequences obtained with mass spectrometry allowed identification of the coding sequence. The gene encodes a 32 kDa protein, designated Six1 for s ecreted i n x ylem 1. The central part of Six1 corresponds to the 12 kDa protein found in xylem sap of infected plants. A mutant that had gained virulence on a tomato line with the I‐3 resistance gene was found to have lost the SIX1 gene along with neighbouring sequences. Transformation of this mutant with SIX1 restored avirulence on the I‐3 line. Conversely, deletion of the SIX1 gene in a wild‐type strain results in breaking of I‐3 ‐mediated resistance. These results suggest that I‐3 ‐mediated resistance is based on recognition of Six1 secreted in xylem vessels.