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Direct evidence for active segregation of oriC regions of the Bacillus subtilis chromosome and co‐localization with the Spo0J partitioning protein
Author(s) -
Lewis Peter J.,
Errington Jeffery
Publication year - 1997
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1997.mmi530.x
Subject(s) - biology , bacillus subtilis , chromosome , chromosome segregation , dna , genetics , mitosis , microbiology and biotechnology , bacteria , gene
We have developed methods for labelling regions of the Bacillus subtilis chromosome with the nucleotide analogue 5‐bromodeoxyuridine (BrdU) and for subcellular visualization of the labelled DNA. Examination of oriC ‐labelled chromosomes in outgrowing spores has provided direct evidence for active segregation of sister chromosomes. Co‐immunodetection of Spo0J and BrdU‐labelled DNA has directly confirmed the expected close association between this chromosome partitioning protein and the oriC region of the chromosome. The results provide further support for the notion that bacterial cells use an active mitotic‐like mechanism to segregate their chromosomes.