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The AngR protein and the siderophore anguibactin positively regulate the expression of iron‐transport genes in Vibrio anguillarum
Author(s) -
Chen Qian,
Wertheimer Anne M.,
Tolmasky Marcelo E.,
Crosa Jorge H.
Publication year - 1996
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1996.tb02662.x
Subject(s) - vibrio anguillarum , siderophore , biology , gene , transcription (linguistics) , transcription factor , microbiology and biotechnology , limiting , vibrio , virulence , regulator , biochemistry , genetics , bacteria , mechanical engineering , linguistics , philosophy , engineering
Summary Vibrio anguillarum virulence is associated with the presence of a piasmid‐mediated iron‐uptake system expressed under iron‐limiting conditions, which consists of the siderophore anguibactin and specific iron‐transport proteins. This system is maximally expressed under iron‐limiting conditions and requires the AngR protein that acts as a positive regulator of anguibactin biosynthesis and also possess an EntE‐like enzymatic function that may play a role in anguibactin biosynthesis. In this work, we demonstrate that in addition to possessing these functions related to anguibactin production, AngR also positively regulated transcription of the iron‐transport genes fatA and fatB. We also show that transcription of angR is repressed by Fur under iron‐rich conditions. In addition, we present evidence that anguibactin itself enhanced transcription of the iron‐transport genes fatA and fatB , independently of AngR and the frans‐acting factor (TAF) product(s). The presence of either AngR (together with the TAF product(s)) or anguibactin alone led to a partial level of expression of the iron‐transport genes fatA and fatB , while full expression is achieved when AngR, the TAF products and anguibactin are all present.