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Construction of a ferritin‐deficient mutant of Campylobacter jejuni : contribution of ferritin to iron storage and protection against oxidative stress
Author(s) -
Wai Sun Nyunt,
Nakayama Koji,
Umene Kenichi,
Moriya Tetsuhiro,
Amako Kazunobu
Publication year - 1996
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1996.tb02633.x
Subject(s) - ferritin , biology , campylobacter jejuni , mutant , oxidative stress , microbiology and biotechnology , open reading frame , gene , campylobacter , paraquat , biochemistry , genetics , bacteria , peptide sequence
Summary The ferritin‐encoding gene (cft) of Campylobacter jejuni was cloned and sequenced. The nucleotide sequence of cft had a 501 bp open reading frame for a protein with 167 amino acids and a predicted molecular mass of 19180 Da, and showed a high similarity to that of Helicobacter pylori and Escherichia coli ferritin genes. To determine the biological function of ferritin in C. jejuni , a ferritin‐deficient mutant was constructed. The growth of ferritin‐deficient strain SNA1 was clearly inhibited under iron deprivation. The ferritin‐deficient mutant was more sensitive to killing by H 2 O 2 and paraquat than the isogenic parent strain. These findings demonstrate that ferritin in C. jejuni makes a significant contribution to both iron storage and protection from intracellular iron overload, and resulting iron‐mediated oxidative stress.