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Regulation by low temperatures and anaerobiosis of a yeast gene specifying a putative GPI‐anchored plasma membrane
Author(s) -
Donzeau Mariel,
Bourdineaud JeanPaul,
Lauquin Guy J.M.
Publication year - 1996
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1996.tb02631.x
Subject(s) - biology , gene , repressor , saccharomyces cerevisiae , start codon , gene expression , yeast , hspa14 , biochemistry , microbiology and biotechnology , nucleotide , heat shock protein , hsp70
Summary Expression of the yeast Saccharomyces cerevisiae SRP1 (serine‐rich protein) gene is shown here to be induced both‐ by low temperature and anaerobic growth conditions. We show that anaerobic SRP1 expression is haem‐dependent; however, haem influence does not operate through the action of the hypoxic‐gene ROX1 repressor. The SRP1 promoter region displaying the stress‐responsive elements is restricted to its first 551 bp, upstream of the initiation codon, although an upstream activation site contained in upstream sequences is required for full promoter activity. In addition, we demonstrate that the TIP1 gene, sharing similar nucleotide and polypeptide structure with SRP1 , and previously reported to be a cold‐shock‐inducible gene, is also a hypoxic gene. Srp1 protein production is similarly induced by low temperature and anaerobic growth conditions. This protein, detected in the plasma membrane fraction, is shown to be exposed on the cell surface via a glycosyl‐phosphatidylinositol membrane anchoring.