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Countertranscript‐driven attenuation system of the pAMβ1 repE gene
Author(s) -
Chatelier E.,
Ehrlich S. D.,
Jannière L.
Publication year - 1996
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1996.tb02550.x
Subject(s) - biology , plasmid , repressor , transcription (linguistics) , gene , mutagenesis , genetics , microbiology and biotechnology , enterococcus faecalis , transcription factor , mutation , escherichia coli , linguistics , philosophy
Summary The plasmid‐encoded RepE protein is absolutely essential and rate‐limiting for replication of the promiscuous plasmid pAMβ1 originating from Enterococcus faecalis. We previously showed that the rep gene is transcribed from a promoter that is negatively regulated (10‐fold reduction) by the CopF repressor. In this report, we show that this transcription is decreased a further 10‐times by a countertranscript‐driven transcriptional attenuation system. Extensive mutagenesis revealed that this system operates by a mechanism similar to that previously described for the unrelated repC gene of plasmid pT181.