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Induction of haemolytic activity in Escherichia coli by the slyA gene product
Author(s) -
Oscarsson Jan,
Mizunoe Yoshimitsu,
Uhlin Bernt Eric,
Haydon David J.
Publication year - 1996
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1996.tb02500.x
Subject(s) - biology , cytolysin , escherichia coli , gene , plasmid , gene product , genetics , microbiology and biotechnology , gene expression , virulence
Summary The Salmonella typhimurium protein SlyA ST , originally described as a cytolysin, shows sequence similarities to several known bacterial regulatory proteins. A homologue to the slyA St gene has been localised to min 37 of the Eschericia coli K‐12 chromosome and has been designated slyA EC When introduced in trans on a plasmid, the slyA EC gene conferred a haemolytic phenotype on wild‐type but not clyA ‐knockout strains of E. coli K‐12. The clyA gene encodes a novel haemolysin that is not expressed by wild‐type E. coli under tested laboratory conditions. Western and Northern blot analyses, and DNA‐band‐shift assays support a model whereby the SlyA EC protein activates clyA expression by binding to the clyA promoter region, thereby supporting the sequence similarity data in suggesting that SlyA ST is a haemolysin activator rather than being a haemolysin per se .