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Characterization and distribution of two insertion sequences, IS 1191 and iso‐IS 981 , in Streptococcus thermophilus : does intergeneric transfer of insertion sequences occur in lactic acid bacteria co‐cultures?
Author(s) -
Guédon G.,
Bourgoin F.,
Pébay M.,
Roussel Y.,
Colmin C.,
Simonet J. M.,
Decaris B.
Publication year - 1995
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1995.tb02392.x
Subject(s) - biology , streptococcus thermophilus , genetics , insertion sequence , inverted repeat , lactococcus lactis , direct repeat , nucleic acid sequence , open reading frame , sequence (biology) , transposable element , peptide sequence , lactobacillus , genome , bacteria , dna , gene , lactic acid
Summary A chromosomal repeated sequence from Streptococcus thermophilus was identified as a new insertion sequence (IS), IS 1191. This is the first IS element characterized in this species. This 1313 bp element has 28 bp imperfect terminal inverted repeats and is flanked by short direct repeats of 8bp. The single large open reading frame of IS 1191 encodes a 391‐amino‐acid protein which displays homologies with transposases encoded by IS 1201 from Lactobacillus helveticus (44.5% amino‐acid sequence identity) and by the other ISs of the IS 256 family. One of the copies of IS 1191 is inserted into a truncated iso‐IS981 element. The nucleotide sequences of two truncated iso‐IS 981 s from S. thermophilus and the sequence of IS 981 element from Lactococcus lactis share more than 99% identity. The distribution of these insertion sequences in L. lactis and S. thermophilus strains suggests that intergeneric transfers occur during co‐cultures used in the manufacture of cheese.