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The enterotoxin gene ( cpe ) of Clostridium perfringens can be chromosomal or plasmid‐borne
Author(s) -
Cornillot Emmanuel,
SaintJoanis Brigitte,
Daube Georges,
Katayama Seiichi,
Granum Per Einar,
Canard Bruno,
Cole Stewart T.
Publication year - 1995
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1995.tb02373.x
Subject(s) - biology , clostridium perfringens , transposable element , gene , enterotoxin , genetics , open reading frame , plasmid , locus (genetics) , genomic dna , microbiology and biotechnology , mutant , escherichia coli , peptide sequence , bacteria
Summary The location of the cpe gene, encoding the enterotoxin responsible for food poisoning in humans, has been studied in a series of enterotoxigenic Ciostridium perfringens strains by means of pulsed field gel electrophoresis of genomic DNA. The cpe gene was found at the same chromosomal locus in strains associated with food poisoning in humans and was shown to be linked to a repetitive sequence, the Hin dlll repeat, and an open reading frame, ORF3, that may be part of an insertion sequence. In contrast, when the strains originated from domesticated livestock cpe was located on a large episome where it was often close to a copy of the transposable element IS 1151. In these cases, the Hin dlll repeat was not linked to the cpe gene although this was generally preceded by ORF3.