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The Escherichia coli DnaK chaperone machine and bacteriophage Mu iate transcription
Author(s) -
Sand Olivier,
Desmet Lucie,
Toussaint Ariane,
Pato Martin
Publication year - 1995
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1995.tb02366.x
Subject(s) - biology , transcription (linguistics) , chaperone (clinical) , mutant , escherichia coli , bacteriophage , promoter , microbiology and biotechnology , transcription factor , dna binding protein , genetics , gene , gene expression , medicine , philosophy , linguistics , pathology
Summary Bacteriophage Mu does not grow on temperature‐sensitive E. coli dnaK mutants at elevated temperatures because of a defect in late transcription. As the Mu‐encoded C protein is required for activation of transcription from the phage late promoters, we attempted to determine if DnaK and its accessory proteins DnaJ and GrpE are required for synthesis of C protein or at a later step. We found that the chaperones act in Mu late transcription beyond C‐protein synthesis, and that C‐protein stability is decreased in the mutant hosts. This suggests that the DnaK chaperone machine may be required for the proper folding and/or multi‐merization of C protein.