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Insertion of inverted Ter sites into the terminus region of the Escherichia coli chromosome delays completion of DNA replication and disrupts the cell cycle
Author(s) -
Sharma Bela,
Hill Thomas M.
Publication year - 1995
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1995.mmi_18010045.x
Subject(s) - biology , dna replication , control of chromosome duplication , origin recognition complex , genetics , ter protein , origin of replication , semiconservative replication , cell division , eukaryotic dna replication , microbiology and biotechnology , regulon , dna , gene , escherichia coli , cell
To investigate the co‐ordination between DNA replication and cell division, we have disrupted the DNA replication cycle of Escherichia coli by inserting inverted Ter sites into the terminus region to delay completion of the chromosome. The inverted Ter sites (designated Inv Ter :: spc r ) were initially inserted into the chromosome of a Δ tus strain to allow unrestrained chromosomal replication. We then introduced a functional tus gene by transforming the Inv Ter :: spc r strain with a plasmid carrying the tus gene under control of an arabinose‐inducible promoter. In the presence of 0.2% arabinose, the cells formed long filaments, suggesting that activation of the inverted Ter sites by Tus arrested DNA replication and delayed the onset of cell division. Induction of sfiA , a gene in the SOS regulon, was observed following arrest of DNA replication; however, when a sfiB114 allele was introduced into Inv Ter :: spc r strain, long filaments were still formed, suggesting that the sfi ‐independent pathway also caused filamentation. Either recA :: cam r or lexA3 alleles suppressed filamentation when introduced in the Inv Ter strain. Interestingly, in both the recA :: cam r and lexA3 mutants, virtually all cells had a nucleoid, suggesting that cell division was proceeding even though DNA replication was not complete. These results suggest that DNA replication and cell division are uncoupled when recA is inactivated or when genes repressed by LexA cannot be induced.