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Transcriptional regulation of repressor synthesis in mycobacteriophage L5
Author(s) -
Nesbit Chadd E.,
Levin Margaret E.,
DonnellyWu Mary K.,
Hatfull Graham F.
Publication year - 1995
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1995.mmi_17061045.x
Subject(s) - biology , repressor , computational biology , transcriptional regulation , genetics , microbiology and biotechnology , gene , transcription factor
Mycobacteriophage L5 is a temperate phage of the mycobacteria that forms stable lysogens in Mycobacterium smegmatis . Lysogeny is maintained by the putative repressor, the gene 71 product, which also mediates immunity to superinfection. We show here that there are three promoters located upstream of gene 71 which are active in an L5 lysogen but which do not require any phage‐encoded proteins. In early lytic growth, gene 71 is also transcribed from a promoter, P left , located at the right end of the genome and which appears to be a target of gp71 regulation. A model is given for the regulation of L5 life cycles.