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NusG overexpression inhibits Rho‐dependent termination in Escherichia coli
Author(s) -
Burova Elena,
Gottesman Max E.
Publication year - 1995
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1995.mmi_17040633.x
Subject(s) - biology , escherichia coli , transcription (linguistics) , microbiology and biotechnology , in vivo , in vitro , transcription factor , biophysics , biochemistry , gene , genetics , philosophy , linguistics
The Escherichia coli NusG protein binds Rho in vitro and is required for efficient Rho‐dependent transcription termination in vivo . In this work we examine transcription termination in cells that overexpress NusG. Termination at the Rho‐dependent λtL1 and λtR1 sites was significantly inhibited by excess NusG, whereas the Rho‐independent λtl site was fully functional. Although Western analysis showed no reduction in the levels of soluble Rho, termination was restored when Rho was also overexpressed. Our data indicate that the ratio of NusG and Rho proteins affects the efficiency of transcription termination.