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Gradient of genomic diversity in the Pseudomonas aeruginosa chromosome
Author(s) -
Römling Ute,
Greipel Joachim,
Tümmler Burkhard
Publication year - 1995
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1995.mmi_17020323.x
Subject(s) - biology , pseudomonas aeruginosa , chromosome , diversity (politics) , genetics , computational biology , microbiology and biotechnology , bacteria , gene , sociology , anthropology
In 545 Pseudomonas aeruginosa strains, mainly collected from patients with cystic fibrosis, Spel‐Dral macrorestriction fragment lenght diversity was scanned for using probes of known map position on th P.earuginosa PAO chromosome. Southern analysis of the 60 unrelated clones uncovered a gradient of macrorestriction fragment lenght polymorphisms (RFLPs) from the origin of replication towards the auxotroh‐poor region of the P. aeruginosa population in the region encompassed by the rrn operons. The oriC ‐reactive Spel fragment was conserved in nearly all isolates examined. Few fragment lenght classes were seen for the alga60‐, algR‐ and toxA ‐encoding Spel fragments. Fragment siz varied within one class by up to 20 kb. Two probes from the auxotroph‐poor region detected a broad size range for the Spel fragment, suggestiong extensive genomic deversity in these reions. Subclonalvariation of fragment size was detected at all investigated loci in at least one of the analysed clones, but within one particlular clone, Spel ‐RFLPs were found at only few loci.