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Inducible transposition in Streptomyces lividans of insertion sequence IS 6100 from Mycobacterium fortuitum
Author(s) -
Smith Barry,
Dyson Paul
Publication year - 1995
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1995.18050933.x
Subject(s) - transposable element , insertion sequence , biology , transposase , transposition (logic) , streptomyces , inverted repeat , genetics , plasmid , mycobacterium fortuitum , gene , mycobacterium , mutant , genome , bacteria , linguistics , philosophy
Transposition of IS 6100 , originally isolated as part of the compound transposon Tn 610 from Mycobacterium fortuitum , was tested in the related actinomycete Streptomyces lividans . Cointegrate formation was observed, as expected for this IS 6 ‐related element, and involved apparent random integration of the temperature‐sensitive vector carrying IS 6100 and concomitant duplication of the insertion sequence. This establishes that a single copy of the insertion sequence can promote transposition and is a precedent for the functioning of a heterologous transposable element in Streptomyces . Transposition could be induced 100‐fold by external transcription emanating from a copy of the thiostrepton‐inducible promoter ptipA located outwith the insertion sequence and resulting in overexpression of the transposase gene. Thus, in constrast to other prokaryotic transposable elements, IS 6100 appears to have no effective means of protecting itself from external activation.