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Identification of a novel sugar‐H + symport protein, FucP, for transport of L‐fucose into Escherichia coli
Author(s) -
Gunn Frank J.,
Tate Christopher G.,
Henderson Peter J. F.
Publication year - 1994
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1994.tb01066.x
Subject(s) - symporter , biology , fucose , biochemistry , escherichia coli , microbiology and biotechnology , membrane transport protein , transporter , glycoprotein , gene
Summary l ‐Fucose (6‐deoxy‐ l ‐galactose) is used as sole carbon source by many microorganisms, and its transport into Escherichia coli is mediated by An l ‐fucose‐H + symport activity, in order to determine the nature of a putative transporter encoded by the E. coli fucP gene and Identify its protein product it was cloned downstream of the inducible T7 RNA polymerase and lambda O l P l promoters, induction of the T7 promoter resulted in the expression of [ 14 C]‐ l ‐fucose uptake activity and the concomitant expression of a [ 35 S]‐Met‐labelled 32 kDa protein at levels too tow for detection by staining with Coomassie briiiiant blue or for protein sequencing, induction of the lambda O l P l promoter caused the appearance of l ‐fucose‐H + symport activity and of a Coomassie brilliant blue‐stained 32 kDa membrane protein expressed at high levels sufficient for identification as FucP by N ‐terminal protein sequencing. The FucP protein is, therefore, a sugar‐H + symporter different in amino acid sequence from any other known transporter. These and other results illustrate the general unpredictability of cloning strategies for attempting the amplified expression of membrane transport proteins.