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Role of rpoS (katF) in oxyR ‐independent regulation of hydroperoxidase I in Escherichia coli
Author(s) -
Ivanova Anna,
Miller Cathy,
Glinsky Gennadi,
Eisenstark Abraham
Publication year - 1994
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1994.tb01043.x
Subject(s) - rpos , biology , escherichia coli , enterobacteriaceae , microbiology and biotechnology , genetics , gene , gene expression , promoter
Summary We present evidence showing that rpoS (katF) is a regulator of katG gene transcription In an oxyR ‐independent manner. Mutation of the rpoS gene in several different Escherichia coli strains caused a significant reduction in catalase HPI activity. In rpoS δ oxyR double mutants, the level of HPI was considerably lower compared to the δ oxyR parent strain, and was restored when transformed with an rpoS + plasmid. Overproduction of HPI in oxyR suppressor strains was greatly diminished after inactivation of the rpoS gene and was accompanied by a substantial increase in sensitivity to menadione. Beta‐galactostdase expression from a katG::lacZ promoter was lower in rpoS strains compared to rpoS + isogenic parents. Several δ oxyR strains had detectable levels of katG transcription that was significantly diminished after rpoS gene inactivation.