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CooC and CooD are required for assembly of CS1 pili
Author(s) -
Froehlich Barbara J.,
Karakashian Alexander,
Meisen Lawrence R.,
Wakefield Jeffrey C.,
Scott June R.
Publication year - 1994
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1994.tb01028.x
Subject(s) - pilus , biology , pilin , periplasmic space , mutant , open reading frame , complementation , genetics , microbiology and biotechnology , escherichia coli , gene , peptide sequence
Summary Many strains of enterotoxigenic Escherichia coli (ETEC) isolated from patients with diarrhoeal disease exhibit CS1 pili on their surfaces. These appendages, which are thought to be important for colonization of the upper intestine, are composed largely of multiple Identical protein subunits encoded by cooA. We have sequenced the DNA directly downstream of cooA and identified two open reading frames, cooC and cooD , transcribed in the same direction as cooB and cooA. Following cooD Is DNA homologous to an insertion sequence, so cooB , A, C and D appear to encode all the information needed for E. coli K‐12 to synthesize CS1 pili. Complementation analysis of mutants cloned in E. coli K‐12 and constructed in an ETEC‐derived strain indicates that cooC and cooD are not required for stability of the major CS1 pilin protein or for its transport to the periplasm, but, like cooB , both are needed for assembly of cooA into pili.