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Modulated expression of promoters containing upstream curved DNA sequences by the Escherichia coli nucleoid protein H‐NS
Author(s) -
Zuber F.,
Kotlarz D.,
Rimsky S.,
Buc H.
Publication year - 1994
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1994.tb01012.x
Subject(s) - promoter , biology , nucleoid , context (archaeology) , dna , gene , escherichia coli , microbiology and biotechnology , binding site , dna binding protein , genetics , gene expression , transcription factor , paleontology
Summary Replacement of the CRP‐binding site of the gal control region by curved sequences can lead to the restoration of promoter strength in vivo. One curved sequence called 5A6A, however, failed to do so. The gene hns exerts a strong negative control on the resulting 5A6A gal promoter as well as on the distant bla promoter, specifically in a 5A6A gal context. The product of this gene, H‐NS, displays a better affinity for this particular insert compared to other curved sequences. Mechanisms by which H‐NS may repress promoters both at short and long distances from a favoured binding site are discussed.